ABSTRACT
Objective:To detect the expression of six miRNAs for miR-146a,miR-224,miR-34c,miR-200a,miR-148b,miR-375 level in primary hepatocellular carcinoma patients tissues and serum,and to explore the correlation between the expression of miRNA and inflammatory factors,in the hope of verification whether circulating miRNA could be used as an ideal blood borne biomarker for early detection of primary liver cancer.Methods:Venous blood samples were collected from patients with primary liver cancer,hepatitis,cirrhosis and healthy controls,cancer tissues and para cancerous tissues were collected from patients with cancer.After the total RNA was extracted,the relative expression levels of miRNA were detected by real-time quantitative PCR,and the relationship between the expression level of miRNA and the expression of serum tumor markers AFP,CEA,CA19-9 and CA125 was detected.The correlation between the expression level of miRNAs and the expression of HBsAg,anti-HBs,HBeAg,anti-HBe,anti-HBc and inflammatory factors IL-12,IL-4,IL-6,IL-10,IFN-γ,TNF-α was detected as well.Results:Compared with the healthy group,the expression of miR-34c,miR-224 and miR-146a in serum and tissues of PHC group was significantly up-regulated,and the expression of miR-200a,miR-148b and miR-375 in serum and tissues of PHC group was significantly down regulated.The difference was statistically significant.There was a regression relationship between HBsAg and serum miR-375 and miR-146a,and miR-375 decreased with the increase of HBsAg expression,while miR-146a increased with the increase of HBsAg.There was a regression relationship between IFN-γ and miR-146a,miR-146a increased with the decrease of IFN-γ expression.The diagnostic ability of miR-375 and miR-146a was greater than that of CA19-9 and AFP.Conclusion:MiR-146a,miR-224,miR-34c,miR-200a,miR-148b,miR-375 in serum and tissue of primary hepatocellular carcinoma are differentially express,among them,the diagnostic ability of miR-375 and miR-146a is better than that of AFP and CA19-9,and serum miR-375 and miR-146a may be the new markers for early diagnosis of PHC.